TABLE 2.

Relative amino acid effects on EcoLrp control of PlivK-lacZ

Amino acid added	Relative expression of PlivK-lacZb
	Fig. S1	Fig. 2	Fig. 3 or 7
MOPSa	(1.00)	(1.00)	(1.00)
Gln	1.04	0.76	1.25
Pro	0.78	1.02	1.47
Ala	0.54	0.26	ND
Ser	0.53	NDc	0.20
Lys	0.40	0.76	1.29
Hisd	0.24	0.49	0.48
Thrd	0.15	0.43	0.78
Iled	0.12	0.41	0.38, 0.30
Leud	0.03	0.01	0.01
Metd	0.004	0.03	0.03

^aMOPS alone; no amino acid was added.

^bData for EcoLrp in indicated figures, divided by the value with no added amino acids. The experiment for Fig. S1 used triplicate culture tube-grown cells with a single-time assay using a plasmid-based system; that for Fig. 2 used shaken flasks with multiple samples and linear fit versus culture density and with chromosomal genes; those for Fig. 3 and 7 used shaken flasks and linear fit and a plasmid-based system. Fig. S1 is found in the supplemental material.

^cND, not determined.

^dQualitatively consistent results in three independent in vivo analyses.