TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype/phenotype | Reference or source |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild type | 19 |
| PATI2 | LecB mutant strain derived from PAO1; lecB::Gmr | 50 |
| E. coli strains | ||
| DH5α | supE44 Δ(lacZYA-argF)U169 (φ80dlacZΔM15) hsdR1 recA1 endA1 gyrA96 thi-1 relA1 | 61 |
| S17-1 | E. coli 294::[RP4-2 (Tc::Mu) (Km::Tn7)] pro res recA tra+ Tpr Smr | 42 |
| Plasmids | ||
| pBBC2 | pBBR1MCS containing a 398-bp XbaI/SacI fragment with lecB derived from pEC2 | 50 |
| pET22b | T7 expression vector for E. coli; PelB signal sequence, Apr | Novagen |
| pBBR1MCS | lacZα Cmrrep mob | 24 |
| pBBN22A | pBBR1MCS containing a 398-bp XbaI/SacI fragment bearing the lecB(N22A) genea | This study |
a
lecB(N22A) encodes LecB with an inactivated N glycosylation site.