TABLE 2.

d-Alanine requirements for different alr/dadX-deficient strains

Strain	Growth on selective LB mediuma
	Km+/d-ala−	Km+/d-ala+	Km−/d-ala−	Km−/d-ala+
BL21(DE3)ΔalrΔdadX	N	N	N	V
BL21(DE3)ΔalrΔdadX metJ(R42C)-Kmr	V	V	V	V
BL21(DE3)ΔalrΔdadXPR	N	N	V	V
BL21(DE3)ΔalrΔdadXPRmetJ(R42C)-Kmr	V	V	V	V
BL21(DE3)ΔalrΔdadXPRmetJ-Kmr	N	V	N	V
BL21(DE3)ΔalrΔdadXΔmetC	N	N	N	V
BL21(DE3)ΔalrΔdadXPRΔmetC	N	N	N	V

^aKm, kanamycin; d-ala, d-alanine; +, in the presence of reagent; −, in the absence of reagent; V, viable; N, not viable.