FIG. 1.

Pig production and pigA-O transcription are reduced in a pigS mutant. (A) Pig production by the WT (diamonds) or a pigS mutant strain (HSPIG26) (unfilled squares) throughout growth. (B) Pig production by the WT and a pigS mutant (HSPIG26) in the presence of empty vector control pQE-80L and Pig production by a pigS mutant (HSPIG26) in the presence of pTA27, encoding native PigS at late exponential phase (9 h), are shown. (C) β-Glucuronidase activity was measured from a chromosomal pigA::uidA fusion in an otherwise WT background (KFAAG6) (diamonds) or in a pigS mutant background (TG70) (unfilled squares) throughout growth. Solid lines represent either Pig or β-glucuronidase assays, and dashed lines represent bacterial growth. Data shown are the means ± SD of the results of three independent experiments.