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. 2011 Jan 7;193(5):1212–1221. doi: 10.1128/JB.01130-10

TABLE 3.

Comparative transcriptome analysis of C. glutamicum WT and the ΔhrrA mutanta

Gene ID Gene name Annotation ΔhrrA/wild-type mRNA ratiob
Heme FeSO4
Two-component systems
    cg3247 hrrA Two-component system, response regulator 0.02 0.02
    cg2200 cgtR8 Two-component system, response regulator 1.79 2.13
    cg2201 cgtS8 Two-component system, signal transduction histidine kinase 1.99 2.42
Heme transport/utilization
    cg0466 htaA Secreted heme transport-associated protein 0.46 1.02*
    cg0467 hmuT Hemin-binding periplasmic protein precursor 0.57 1.03*
    cg0468 hmuU Hemin transport system, permease protein 0.60 1.04*
    cg0469 hmuV Hemin transport system, ATP-binding protein 0.86* 1.24*
    cg0470 htaB Secreted heme transport-associated protein 9.00 8.71*
    cg0471 htaC Secreted heme transport-associated protein 8.06 5.09
    cg2202 hrtB ABC-type transport system, permease component 10.90 40.79
    cg2204 hrtA ABC-type transport system, ATPase component 10.47 43.93
    cg2445 hmuO Heme oxygenase 0.06 0.12
    cg3156 htaD Secreted heme transport-associated protein 0.26 0.63*
Heme-containing proteins
    cg0645 cytP Cytochrome P450 0.20 0.32
    cg2403 qcrB Cytochrome b, membrane protein 0.35 0.47
    cg2404 qcrA Rieske iron-sulfur protein 0.36 0.47
    cg2405 qcrC Cytochrome c1 0.36 0.48
    cg2406 ctaE Cytochrome aa3 oxidase, subunit 3 0.34 0.46
    cg2780 ctaD Cytochrome aa3 oxidase, subunit 1 0.51 0.62
    cg3141 hmp Flavohemoprotein 2.74 1.95
    cg3369 Rieske-type iron-sulfur protein 0.57 0.75*
Heme biosynthesis
    cg0497 hemA Glutamyl-tRNA reductase 1.77* 1.44*
    cg0498 hemC Porphobilinogen deaminase 1.62* 1.19*
    cg0516 hemE Uroporphyrinogen decarboxylase 1.87 1.99
    cg0517 hemY Protoporphyrinogen oxidase 1.90* 2.04
    cg0518 hemL Glutamate-1-semialdehyde 2,1-aminomutase 1.77 2.10
    cg0519 Putative phosphoglycerate mutase 1.59* 1.74
    cg0520 Secreted thiol-disulfide isomerase or thioredoxin 1.51* 1.70
    cg0522 ccsA Cytochrome c biogenesis protein membrane protein 1.34* 1.53
    cg0523 Membrane protein required for cytochrome c biosynthesis 1.37* 1.75
    cg0524 ccsB Cytochrome c assembly membrane protein 1.39* 1.90
    cg1734 hemH Ferrochelatase precursor 2.41* 2.20
a

This table includes all genes with a putative heme/iron-related function that are differentially expressed in the ΔhrrA mutant. A list of all genes with a ≥2-fold-altered mRNA level is provided in Table S2 in the supplemental material. Genes and operons shown to be directly regulated by HrrA have all been included in this table, irrespective of their relative expression.

b

Average ratio of genes showing a ≥2-fold-altered mRNA level (ΔhrrA/wild-type mRNA ratio) of C. glutamicum cells grown on CGXII minimal medium with 2.5 μM heme or 2.5 μM FeSO4 as the iron source. The mRNA ratios represent average values from three independent DNA microarray experiments (P ≤ 0.05). *, P > 0.05.