FIG. 6.

Analysis of the additional low-complexity sequence in AlpW. (A) Superposition of the 3D structure representation of the native AlpW (yellow; monomer) and the homologous pseudoautoregulator receptor CprB (blue; dimer), the structure of which was determined by X-ray crystallography (34). (B) Comparative DNA binding analysis of AlpW and AlpW-loop by EMSA. The DIG-labeled probe alpVp (386 bp) bearing the AlpW recognition sequence AREV was incubated in the absence (lane 1) or presence of soluble extracts containing recombinant AlpW (lanes 2 and 4) or AlpW-loop (lanes 3 and 5). Concentrated extracts containing the AlpZ-binding ligand were added to the mixture (lanes 4 and 5).