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DNase I footprinting of the promoter region of the pipX gene. DNase I protection assays were carried out with purified NtcA (90 or 180 nM) and a DNA fragment of the pipX promoter region amplified by PCR using oligonucleotides PX18 (unlabeled) and PX19 (32P labeled), in the presence or absence of 0.6 mM 2-OG. The sequence of the NtcA-protected region, including the GTA/GAC triplets (underlined), is indicated.
