FIG. 6.

Effect of constitutive expression of local regulators on icl1 and gltA1 hypoxic response in sigma factor mutants. Derivatives of plasmid pMP167 expressing rv0465c or rv1129c under the control of the constitutive groEL promoter (indicated as pRv0465c and pRv1129c) were introduced in sigB or sigE knockout mutant strains. Cultures were harvested at daily intervals, and mRNA enumeration by qPCR was conducted as described in the Fig. 1 legend. Each panel presents data for one gene (panel A, icl1; panel B, gltA1). The results shown represent the mid-log time point and the time points corresponding to peak gene induction (day 3 for icl1 and day 4 for gltA1).