FIG. 1.

Identification of the genetic locus for fucose utilization. (A) Schematic representation of the chromosomal region associated with the Fuc⁺ phenotype in NCTC 11168. Solid arrows depict open reading frames. Vertical bars indicate locations of frameshift mutations resulting in premature translational termination. The location of the ΔfucP::cat mutation is indicated by the arrow above fucP. ORFs used to complement the fucP mutant are indicated by braces below each ORF. The strain designation for each complementing construct is labeled under each respective brace. The diagonal hatch marks indicate that the entire length of the ORF is not shown. (B) RT-PCR analysis of gene transcription in the wild-type strain and the ΔfucP::cat strain complemented with fucP (CjWM226a). RT+, reverse transcribed with SuperScript RT; RT−, no-SuperScript RT control. Polar effects on transcription of the genes downstream of fucP are evident by the absence of PCR bands in the RT+ lanes for cj0487 and aldA in CjWM226a.