FIG. 5.
RT-PCR analysis of fucose induction of gene expression in C. jejuni NCTC 11168. (A) Expression of dapA, fucP, and aldA in wild-type NCTC 11168 in defined medium supplemented with fucose (50 mM), pyruvate (20 mM), or a mixture of aspartate, glutamate, proline, and serine (DEPS). RT+, RT-PCR with SuperScript RT; RT−, no-SuperScript RT control. (B) Dose-dependent induction of fucP by fucose. Rinduced represents the relative transcript abundance in cultures induced with fucose compared to that in uninduced cohorts. Error bars indicate standard errors from four independent experiments. Values above error bars indicate P values determined by a one-sample t test.