TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description or relevant genotypea | Source or reference |
|---|---|---|
| E. coli strain DH5α | Plasmid propagation strain | Invitrogen |
| C. jejuni strains | ||
| NCTC 11168 | Wild-type C. jejuni | 36 |
| 81116 | Wild-type C. jejuni | 37 |
| 81-176 | Wild-type C. jejuni | 6 |
| CjWM114a | NCTC 11168 derivative, ΔfucP::cat Cmr | This study |
| CjWM226a | CjWM114a derivative, ΔfucP::cat rrs::fucP Cmr Kanr | This study |
| CjWM230a | CjWM114a derivative, ΔfucP::cat rrs::fucP-cj0487 Cmr Kanr | This study |
| CjWM231a | CjWM114a derivative, ΔfucP::cat rrs::fucP-cj0488 Cmr Kanr | This study |
| CjWM227a | CjWM114a derivative, ΔfucP::cat rrs::fucP-aldA Cmr Kanr | This study |
| Plasmids | ||
| pGEM-T | Cloning vector | Promega |
| pUOA18 | E. coli-C. jejuni shuttle vector | 50 |
| pMW10 | Promoterless lacZ plasmid | 51 |
| pfucP | pGEM-T::fucP | This study |
| pWM10 | ΔfucP::cat Cmr, suicide vector to construct CjWM114a | This study |
| pRR | pGEM-T::rrs | 24 |
| pRRK | pRR::aphA3 | This study |
a
Cmr, chloramphenicol resistant; Kanr, kanamycin resistant.