TABLE 2.
Primers used in this study
| Primer name | Primer sequence (5′ → 3′)a |
|---|---|
| fucP_L | TGACCAAAGAATGTTTGCCTT |
| fucP_R | CCACTGGATAATTGCTCCCA |
| ifucP_L | CGCAGATCTCATTAAAACTTTGCGCAGCA |
| ifucP_R | CGCAGATCTATTTATGCTAGGGCGCATTG |
| cat_L | CGCAGATCTTTCCGTCGTCGGTATCGTAT |
| cat_R | CGCAGATCTGTGCGACAAACTGGGATTTT |
| rrs | CTGGAACTCAACTGACGCTAAG |
| rrl | CTCTTGCACATTGCAGTCCTAC |
| aphA3_L | CGCCAATTGCCACAATGATAGAACCAACGA |
| aphA3_R | CGCCAATTGATAGGCAGCGCGCTTATCA |
| KIfucP_L | CGCTCTAGAGGGCTTGTGCTTTTGCTAAG |
| KIfucP_R | CGCTCTAGATGCTCGTGTGCATTACTTCC |
| KIfucP_R4 | CGCCCTAGGTCATCTCACAAACCCCTTCA |
| KIfucP_R5 | CGCCCTAGGGCGCTAGCTACTCTTGAAATCAC |
| KIfucP_R3 | CGCCCTAGGGCAATGGCACCTTTGTTTTT |
| qfucP_L | GGCTTTTAGCGCAGTTTTTG |
| qfucP_RT | CAATGCGCCCTAGCATAAAT |
| cjr01_L | TCCCAGTTCGGATTGTTCTC |
| cjr01_RT | GTACAAGACCCGGGAACGTA |
| pdapA_R | GCAAAGCAAAACCCATAGGA |
| tdapA_L | GCATAGGTGGAGTTTTTCGAG |
| ald_L | CCCACATAATGGAGAATTTATCG |
| ald_R | TTCGGCCGTATAATCCATATAA |
| pcj0487_L | GACGATGAAAAATTAGAGCA |
| pcj0487_R | TGCGAAAAACCATCAGGAAT |
| tcj0488_L | CAGACATGGCTAAAATGGCA |
| pcj0488_R | TTTCCACCATTTTTGTGTGC |
| cj0486FWD | GATAGAGCATTAAATTGGGATG |
| cj0486REV | CCTATAAAGCCATACCAAGCC |
| ggt_L | GGCGGAATTGCCATAGATGC |
| ggt_R | AGTGCCGTAGCGATCAAGCA |
| rpoC | GAACTTGCTATTGCTGAGCC |
| rpsL | ACCCTAGTGCAAACTCCCCT |
a
Added restriction endonuclease sites are underlined.