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. 2008 Dec;89(Pt 12):2923–2932. doi: 10.1099/vir.0.2008/006254-0

Fig. 2.

Fig. 2.

The H5N1 3P complex is more active than the WSN 3P complex in both cap-independent and cap-dependent transcription. (a) Purified 3P complexes from the VN1203 (H/H/H) and WSN (W/W/W) viruses, along with chimeric (H/H/W and W/W/H) and dimeric complexes (H/H; negative control), were detected by silver staining and immunoblotting against PB2 (top and bottom panel, respectively). PB2-normalized complexes were used in functional assays (30 °C). (b) The complexes were analysed for cap-independent (ApG) or cap-dependent transcription (top and bottom panel, respectively). The transcription products in the ApG-primed assay (14 nt long transcript) and in the globin-primed assay (triplet band; capped primer +14 nt) were detected by autoradiography. (c, d) Quantitative data for the ApG- and the cap-RNA-primed assays are shown. The data were normalized in terms of fold activity over the activity of the W/W/W complex. The results shown represent mean transcriptional activity data from at least three independent assays. Bars, sem.