Fig. 3.

The human H5N1 3P complex is active at high temperatures and the H5N1 PB2 subunit confers this same phenotype on the WSN 3P complex. Equivalent amounts of polymerase complex (as determined by polymerase activity at 30 °C) were tested for transcriptional activity at different temperatures (30, 34, 37, 39, 42 °C). (a, b) Representative autoradiograms and quantitative data (top and bottom panel, respectively) for the ApG- (a) and the cap-RNA-primed (b) assays are shown. The data were normalized in terms of percentage of the amount of fully extended product at 30 °C (as quantified by Phosphorimager analysis); the results shown represent mean transcriptional activity data from at least four independent assays. Bars, sem.