FIG. 4.
β-Galactosidase activities of VirAperi insertion mutants. The graph depicts results of a representative yeast two-hybrid assay to characterize the interactions of the VirA periplasmic domain with VirAperi mutations. Liquid assays for β-galactosidase activity of yeast strains carrying a wild-type VirAperi bait domain and various insertion mutants of the periplasmic domain were performed to study interaction as described in the Materials and Methods. Activity is expressed in Miller units. See the text for a description of the results. This assay was performed in triplicate, and similar results were observed in at least two independent experiments. neg, negative control.