FIG. 7.
TM2 mutants. A348-3 strains carrying the PvirB::lacZ reporter fusion on pSW209Ω and wild-type virA or the TM2 R259S and L276R single mutants or the R259S/L276R double mutant on the plasmid pAW50 were grown at 25°C in pH 5.5 induction medium with 10 mM glucose (a) or glycerol (b), at increasing concentrations of the phenolic inducer acetosyringone (AS). β-Galactosidase activity was determined after 20 h. Results are reported in Miller units. Samples were assayed in triplicate, and results are plotted as means with standard deviations. Three independent assays were performed.