. 2011 Jan 7;193(6):1436–1448. doi: 10.1128/JB.01227-10

TABLE 2.

Effect of ChvE on AS sensitivity and maximal activity of VirA as a function of β-galactosidase activity

Protein	No ChvE		Wild-type ChvE		ChvE^T187P
Protein	ED₅₀ (μM)^a	Maximal activity^b	ED₅₀ (μM)^a	Maximal activity^b	ED₅₀ (μM)^a	Maximal activity^b
VirA^wt	33	660 ± 92	2	5,717 ± 548	0.3	7,928 ± 188
VirA^E255L	44	950 ± 37	19	6,066 ± 38	4	5,916 ± 351
VirA^N226Y	42	550 ± 33	15	2,340 ± 232	1	7,052 ± 112
VirA^I236F	75	655 ± 212	43	1,531 ± 125	7	4,213 ± 595
VirA^Q229K	108	2,076 ± 362	121	1,807 ± 73	78	4,541 ± 254
VirAⁱ¹⁶⁹	26	2,838 ± 219	19	2,342 ± 15	23	2,190 ± 133

Expressed as ED₅₀, or the micromolar concentration of the phenolic inducer that elicits 50% of the maximal response (calculated as described in Materials and Methods).

β-Galactosidase activities ± standard deviations are expressed in Miller units and represented here as averages (and standard deviations) of at least three experiments.