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Replication origin region of the B. subtilis chromosome. Shown is the site of insertion of the gene for resistance to chloramphenicol (cat) in strain LR166. The promoter for dnaA is marked by an arrow. The essential elements of the origin of replication are shown as white boxes. Primers LRL96 and LRL174 were used to amplify this region, which also includes the genes rpnA and rpmH and portions of the genes spoIIIJ and dnaN.
