TABLE 2.
MCP phenotypes of pdu deletion mutants
| Relevant genotype (strain) | MCP formation | Growth arrest | Doubling time with B12 (h) |
Growth lag on 1,2-PD minimal medium (h) | |
|---|---|---|---|---|---|
| Limiting | Saturating | ||||
| Wild-type S. enterica LT2 | Normal | No | 16.9 ± 0.83 | 5.4 ± 0.11 | 17.4 ± 0.3 |
| ΔpduA (BE464) | Enlarged | Yes | 7.8 ± 0.44 | 4.5 ± 0.25 | 17.5 ± 0.3 |
| ΔpduBB′ (BE213) | Absent, with polar body present | Yes | 4.3 ± 0.10 | 5.2 ± 0.07 | 13.4 ± 0.1 |
| ΔpduJ (BE184) | Elongated or amorphous | Yes | 7.1 ± 0.52 | 4.5 ± 0.11 | 16.1 ± 0.5 |
| ΔpduK (BE770) | Aggregated | No | 16.6 ± 0.37 | 5.6 ± 0.17 | 17.6 ± 0.1 |
| ΔpduN (BE772) | Elongated and varied shapes | Yes | 4.1 ± 0.41 | 5.8 ± 0.16 | 15.9 ± 0.3 |
| ΔpduT (BE885) | Normal | No | 18.0 ± 1.13 | 8.1 ± 0.15 | 18.2 ± 0.3 |
| ΔpduU (BE901) | Normal | No | 16.5 ± 0.60 | 8.2 ± 0.28 | 20.0 ± 0.2 |