TABLE 5.
ZapC contributes to cell division
| Straina | IPTG concn (μM) | Lengthb |
% cells with: |
Total no. of septa | L/S (μm) | ||
|---|---|---|---|---|---|---|---|
| Avg (μm) | SD (μm) | 1 septum | >1 septum | ||||
| TB28 | 0 | 4.4 | 1.1 | 26.5 | 0.0 | 160 | 16.7 |
| CH59 (ΔzapC) | 0 | 4.4 | 1.2 | 25.0 | 0.2 | 152 | 17.4 |
| CH41 (ΔzapA) | 0 | 5.9 | 3.3 | 33.6 | 2.2 | 216 | 16.5 |
| CH63 (ΔzapA ΔzapC) | 0 | 9.5 | 12.4 | 24.8 | 1.3 | 158 | 36.3 |
| CH65 (ΔzapA ΔzapB ΔzapC) | 0 | 9.7 | 9.8 | 17.5 | 0.3 | 108 | 54.3 |
| CH65(iBL3) [ΔzapABC (Plac::zapC)] | 10 | 5.7 | 5.1 | 23.5 | 1.7 | 152 | 22.6 |
| CH65(iBL3) [ΔzapABC (Plac::zapC)] | 50 | 4.8 | 3.0 | 26.5 | 2.3 | 174 | 16.7 |
| CH65(iBL4) [ΔzapABC (Plac::zapC-gfp)] | 50 | 7.8 | 8.1 | 26.1 | 2.2 | 171 | 27.6 |
| CH65(iBL4) [ΔzapABC (Plac::zapC-gfp)] | 200 | 5.0 | 3.5 | 25.3 | 1.7 | 162 | 18.5 |
a
Cells were grown in LB at 30oC with IPTG as indicated to OD600 = 0.6 to 0.8 and chemically fixed before imaging and measurements of cell parameters. Between 600 and 605 cells from each culture were analyzed.
b
Average cell lengths and standard deviations are given.