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Upregulation of GadAB expression upon GadXY overexpression. Cells were grown in LB medium, and samples were taken after 2 and 4 h and analyzed by SDS-PAGE and immunoblotting using antisera against GadAB and Lep. (A) FTL10 and its parental strain MC4100-A; (B) FTL10 containing pGadXY or control vector pBR322.
