FIG. 3.

BamHI-HindIII fragment containing a Cm^r cassette integrated into the chromosome of B. bacteriovorus HD100 used for construction of knockout plasmid pNR16. The fragment was obtained by PCR amplification using primers Bd29 and Bd30 from chromosomal DNA of a saprophytic mutant obtained through transposon mutagenesis. The Cm^r cassette is indicated by white boxes surrounding the cat gene (light gray) and integrated into the chromosomal DNA (black boxes) interrupting the hit gene (Bd0108) shown above the chromosomal region (dark gray boxes). On each site of the integration six nucleotides are duplicated (positions 193 to 198, GGCGCC).