TABLE 3.

Sequence alterations of saprophytic and axenic B. bacteriovorus HD100 mutants

Position	Gene or proteina	Amino acid change (position in gene product)	Sequence alterationc
			HD100-wtb	M1	M1.1	M2	M2.1	M11/Strep
35,765	Bd0040	Unmodified protein	C			T	T
96,997	hit [Bd0108]	Frameshift, C-terminal amino acids modified (64 to 103)						GTAGG
97,076	hit [Bd0108]	Stop codon (37)	C	A	A
97,125	hit [Bd0108]	Frameshift, C-terminal amino acids modified (19 to 100)	C			*	*
170,857	Bd0183	H to N (160)	G			T	T
521,234	citZ [Bd0562]	Frameshift, change of amino acids 261 to 382 (end)	T				*
707,368	Bd0745	P to S (87)	C	T	T
1,331,024	Bd1408	V to A (1)	A			G	G
1,723,594	Bd1797	N to K (288)	T	A
2,317,782	cheY [Bd2409]	D to G (12)	C				A
2,318,005	cheY [Bd2409]	L to F (86)	T	C	C
2,567,148	Bd2644	T to I (420)	C		T
2,876,738	rpsL [Bd2981]	K to R (43)	T					C
2,880,920	rpoC [Bd2983]	E to K (86)	C		T
2,974,067	nifA [Bd3063]	G to D (160)	C				T
3,370,837	rhlB [Bd3461]	Stop codon (461)	G				A
3,374,202	pcnB [Bd3464]	Stop codon (328)	C		T
3,617,768	Intergenic region between Bd3743 and Bd3745		C			A	A
3,704,211 3,704,212	Bd3838	Frameshift, C-terminal amino acids modified (92 to 120)	AA				*

^ahit, hypothetical protein associated with the host (prey) interaction, unknown function; citZ, citrate synthase hypothetical protein; cheY, chemotaxis response regulator; rpsl, 30S ribosomal protein S12; rpoC, DNA-directed RNA polymerase, beta subunit; nifA, transcriptional regulator; rhlB, ATP-dependent RNA helicase; pcnB, poly(A) polymerase; Bd0040, putative radical activating enzyme; Bd1408, outer membrane protein; Bd0183, Bd0745, Bd1797, and Bd2644, hypothetical proteins.

^bThe sequence of wild-type B. bacteriovorus HD100 used in this study.

^c*, deletion.