FIG. 2.

Transcriptional analysis of the mreB-like genes of S. coelicolor by semiquantitative RT-PCR. Cultures of S. coelicolor M145 were grown on cellophane discs on LB agar or MS agar for different time periods. The stage of morphological differentiation is indicated for the different time points. Following RNA isolation and cDNA synthesis, the amounts of transcripts were compared by PCR. The expression of hrdB served as an internal control. The expression of mreB and mbl increased during morphological differentiation. In contrast, sco6166 is highly expressed in vegetative mycelia but no longer is transcribed after the onset of sporulation.