TABLE 3.
Oligonucleotides used for cloning experiments
| Primer | Sequencea (5′→3′) |
|---|---|
| Ecoupfw | AAGAATTCTGATCTCCTGCGCCTG |
| uprchBgBc | TCATGATCAGATCTCACGGTCATCGTCCG |
| loupchBcBg | GTGAGATCTGATCATGATCCGGTACCGGG |
| Hlorev | GGAAGCTTGACAGCGTGAGAGAC |
| mblupNde | GGCATATGACCGCCAGTACTG |
| mblloBg-gfp | GGAGATCTGTCGGAGTCGGCGTG |
| 6166upNde | GGCATATGACCGTGCTCCGG |
| 6166loBg | CCAGATCTGTGCGGGTGGGTGTC |
| mblredup | GGCCCCGCACGGTCCGCGCTCTCGGGAGGATTCGCCATG GATATCATTCCGGGGATCCGTCGACC |
| mblredrev | TGCGTCAGGCTTCCCCGGTCGCCGCCCGGCGGCGGATCA GATATCTGTAGGCTGGAGCTGCTTC |
| loupchBcBg | GTGAGATCTGATCATGATCCGGTACCGGG |
| Hlorev | GGAAGCTTGACAGCGTGAGAGAC |
| CHSTEup2 | AGGATTCGCCATGGAATTCCACGCCGACTCCGACTG |
| HKHrev2 | CCAAGCTTGGCGGTCGGGTGCAGG |
| mreBXba | AATCTAGAGAACGACATTGAGTTC |
| mreBK | TCGGTACCGATCTACGGGGCGAGGC |
| mblXba | AATCTAGAGTATTGGCAGTACTG |
| mblK | CTGGTACCCAGTCGGAGTCGGCGTG |
| 6166Xba | ACTCTAGAGACCGTGCTCCGGCGG |
| 6166K | AAGGTACCCAGTGCGGGTGGGTGTC |
| rodZXba | ACTCTAGAGTCCAACGGCAAATCC |
| rodZK | TTGGTACCCATCCGACCTGCGGGT |
| mreCXba | ACTCTAGAGAGGGACACGAAAGAG |
| mreCK | AAGGTACCACGGCTCGGGCTGCTC |
| mreDXba | ACTCTAGAGCGCGTCAACCGGATC |
| mreDK | AAGGTACCACAGCCGCTTGACCCC |
| pbp2Xba | ACTCTAGAGACCAACATCCCCGAG |
| pbp2K | AAGGTACCATACGCGGTGCCCTCC |
| sfrXba | ACTCTAGAGACCGGCAACAGCTTC |
| sfrK | AAGGTACCACGCCGACATCGGTCT |
a
Restriction sites are underlined.