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Protection of DNA integrity. Plasmid pUC19 DNA was irradiated up to 15 kGy in 25 mM PiB, in 0.8 and 3.8 M KCl, and in protein-free UFs of H. salinarum, E. coli, and P. putida (diluted 1:5). DNA topology was analyzed by agarose gel electrophoresis. The first lanes are molecular size ladders. L, linear; SC, supercoiled; OC, open circular plasmid.
