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Gel mobility shift assays. The 297-bp (positions −283 to +14) end-labeled fsrB promoter fragment (A) or the 359-bp (positions −307 to + 52) end-labeled gelE fragment (B) was incubated with FsrA-His6 at the concentrations indicated in the figure. C, complex; FP, free probe; NS, nonspecific DNA; S, specific DNA.
