TABLE 1.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pET28a | Expression vector | Novagen |
| pET-FsrA | pET28 containing E. faecalis fsrA | |
| pML28 | pAT28 plus the aphA3 promoter on a 369-bp EcoRI/BamHI fragment | |
| pDII32 | pML28 expressing E. faecalis FsrA-His6 | |
| pWH1520 | E. coli/B. megaterium shuttle vector | MoBiTec |
| pWH-FsrA | pWH1520 expressing E. faecalis FsrA-His6 | |
| pMAL-c2E | Expression vector | New England Biolabs |
| pMAL-FsrC | pMAL-c2E containing the E. faecalis FsrC cytosolic region | |
| pBluescript IIKS | Phagemid from pUC19 | Stratagene |
| pBlue-fsrA | pBluescript IIKS containing the fsrB promoter region (−351 to +33) | |
| pBlue-fsrB | pBluescript IIKS containing the fsrB promoter region (−283 to +14) | |
| PBlue-gelE | pBluescript IIKS containing the gelE promoter region (−307 to +52) | |
| pCR2.1-TOPO | Cloning vector | Invitrogen |
| pTOPO-fsrB | pCR2.1-TOPO containing the fsrB promoter region (−283 to +14) | |
| pTOPO-gelE | pCR2.1-TOPO containing the gelE promoter region (−305 to +49) |