FIG. 3.

Blue native gel electrophoresis of B. subtilis cells grown to competence. (A) Western blot of whole-cell extracts separated by SDS-PAGE of strains as indicated using ComGC antiserum. (B) Western blot of ComGC in cytosolic and membrane fractions relative to the whole-cell lysate. (C) Western blot of ComGC from 5 to 15% blue-native PAGE of isolated membrane fractions. Membranes were solubilized in Triton X-100 (3%). Triangles indicate ComGC-containing complexes that have entered the gradient gel.