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Localization of competence proteins in spheroplasts. (A) Single ComGA-CFP focus. (B) Cells with two ComGA-CFP foci. (C) Colocalization of ComGA-CFP and ComEC-YFP. The cells were grown to competence using the two-step protocol and were treated with 1 mg/ml (final concentration) lysozyme for 10 min. White bars, 2 μm.
