TABLE 1.
Quantitative analysis of localization patterns of competent proteins
Strain | No. of cells | Fluorescent cells (% of cells) | % of fluorescent cells with: |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
1 focus |
2 foci |
3 focia |
4 foci | >4 foci | |||||||
At the pole | In the cellb | Both at the pole | 1 at the pole, 1 in the cell | Both in the cell | 2 at the pole, 1 in the cell | 1 at the pole, 2 in the cell | |||||
ComGA-CFP | 1,608 | 16.8 | 33.7 | 5.9 | 26.3 | 16.3 | 0.4 | 10.7 | 1.5 | 3.3 | 1.9 |
ComFA-mCherry | 1,497 | 9.8 | 37.0 | 7.5 | 28.8 | 8.9 | 0.0 | 13.7 | 0.7 | 2.7 | 0.7 |
ComGC-FlAsH | 1,117 | 12.5 | 24.3 | 5.0 | 37.1 | 13.6 | 0.0 | 10.0 | 0.0 | 2.9 | 7.1 |
ComEC-YFP | 573 | 28.4 | 31.9 | 4.3 | 41.1 | 13.5 | 0.0 | 4.9 | 1.8 | 1.8 | 0.6 |
ComFC-YFP | 689 | 19.9 | 39.4 | 7.3 | 26.3 | 9.5 | 0.0 | 10.9 | 1.5 | 1.5 | 3.6 |
ComEB-YFPc | 507 | 20.3 | 65.0 | 15.5 | 11.7 | 1.9 | 5.8 | 0.0 | 0.0 | 0.0 | 0.0 |
ComFB-YFP | 1,861 | 8.0 | 39.6 | 8.1 | 28.8 | 15.3 | 0.0 | 5.4 | 0.9 | 1.8 | 0.0 |
Three foci away from the pole were never observed.
“In the cell” means “not at the pole but toward the cell center.”
Fusion is not fully functional.