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Cell-free expression of ldhA, ldhB, and ldhC. Lactate dehydrogenases were expressed as His-tagged proteins by using the RTS 500 E. coli HY kit in the presence of GroE supplement. Purified LdhA, LdhB, and LdhC were visualized by Western blotting using anti-His antibodies. (A) Purified LdhA (34.6 kDa). (B) Lane 1, purified LdhB (34.5 kDa); lane 2, purified LdhC (34.7 kDa). The sizes of the molecular mass markers (in kDa) are indicated on the left.
