TABLE 2.

mRNA levels of genes involved in glucose catabolism and enterotoxin synthesis in B. cereus strains F4810/72, F837/76, and PA relative to those of strain F4430/73^a

Gene	Difference in mRNA level (n-fold)b under the indicated condition
	Anaerobic growth			Aerobic growth
	F4810/72	F837/76	PA	F4810/72	F837/76	PA
Glucose catabolism gene
ldhA	−2.5	−100	−100	−2.0	−100	−100
ldhB	+2.5	+2.0	−5.0	−50	−100	−5.0
ldhC	+3.2	+6.2	+6.5	+2.2	+12.3	+5.4
pfl	−4.0	−1.2	−2.2	−1.4	−3.7	−3.1
pdhA	+1.4	+1.9	+1.6	−1.1	+1.0	+1.0
pta	−5.0	−3.3	−3.8	−2.5	−2.0	+1.5
ackA	−3.3	−2.3	+1.6	−1.7	−1.5	+2.0
adhE	−7.7	−4.8	−7.7	−4.5	−33.3	−3.7
adhA	−1.1	+1.7	+1.7	+2.3	−1.8	+1.1
sdhA	−2.2	+1.0	−2.5	+3.0	+2.9	+2.4
citZ	−100	−100	−16.0	−50.0	−33.0	−2.7
citB	+1.1	−12.5	−10.0	+2.0	+1.5	−2.3
mdh	+1.6	+4.8	+4.9	−1.1	−1.1	+1.2
Enterotoxin gene nhe	−10	−100	−100	−3.1	−500	−14.3
Regulator genes
plcR	+3.0	+1.4	+4.2	+2.9	+1.1	−1.1
resD	+4.0	+3.2	+5.6	+2.2	+1.9	+2.3
fnr	+6.7	+6.2	+7.5	−1.8	+2.4	+1.5
ccpA	−1.4	−1.1	−2.3	−3.6	−1.7	−1.2

^aB. cereus cells were grown under oxic and anoxic conditions in controlled batch cultures (pH 7.2) on MOD medium with 30 mM glucose as the carbon source.

^bEach change (n-fold) represents the mean value of the mRNA levels of one strain sample in relation to that of the F4430/73 sample. For each experiment, two measurements from two independent RNA samples taken from the mid-exponential growth phase (μ_max) of the same culture were analyzed in parallel. Each data point is an average of the results of the combined experiments. Only ratios of ≤−2 and ≥+2 were considered significant (i.e., P ≤ 0.05) according to the precision of the method. + and − indicate up- and downregulation of genes, and significant values for upregulation and downregulation are underlined and boldface, respectively.