TABLE 3.

Results from controlled batch cultures (pH 7.2) of ldhA and pfo mutants and their parent strain, B. cereus F4430/73^a

Growth parameter	Anaerobic growth			Aerobic growth
	WTb	ldhA	pfo	WT	ldhA	pfo
μmax (h−1)	0.9	0.5	1.0	1.4	1.4	1.1
Final biomass (g·liter−1)	0.8	1.0	0.9	2.6	2.5	2.6
Glucose consumption (%)	100	100	100	100	100	100
Yglucose (g of cells·mol of glucose−1)	26	33	30	86	83	86
Maximal specific glucose consumption rate (mmol·mg−1·h−1)	35	15	34	16	17	13
Yields of end products (mol·mol glucose−1)
Lactate	1.50	1.60	1.70	0.03	0.04	NZc
Acetate	0.29	0.26	0.37	1.0	2.0	2.0
Formate	0.34	0.24	0.19	NZ	NZ	NZ
Ethanol	0.06	0.07	0.08	NZ	NZ	NZ
Succinate	0.01	0.01	0.01	NZ	NZ	NZ
Ethanol/acetate	0.21	0.27	0.21	NDd	ND	ND
NADH recoverede	1.1	1.1	1.2	ND	ND	ND
Total Nhe level (U·g−1 of dry cells)	5.30	1.25	4.35	1.41	0.09	1.29

^aCells were grown under N₂ anaerobiosis (pO₂ = 0%) and full aerobiosis (pO₂ = 100%). Data are the means of triplicate measures obtained from two independent cultures. For clarity, standard deviations (below 10%) are not shown.

^bWT, wild-type parent strain B. cereus F4430/73.

^cNZ, yield was below 0.01 mol·mol glucose⁻¹.

^dND, not determined.

^eNADH recovery was calculated as the ratio of pathways producing NADH versus those consuming NADH.