TABLE 4.

Comparison of the mRNA levels of genes involved in glucose catabolism and enterotoxin synthesis in ldhA and pfo mutant strains^a

Gene	Difference in mRNA levels (n-fold)b under the indicated conditions
	Anaerobic growth		Aerobic growth
	ldhA	pfo	ldhA	pfo
Glucose catabolism genes
ldhA	ND	+1.2	ND	+1.5
pfo	−4.0	ND	−1.1	ND
ldhB	+32.0	−3.4	+2.4	+1.1
ldhC	−1.4	+1.2	+1.0	−1.2
pfl	+1.4	−1.1	+2.8	+3.5
pdhA	−1.5	+1.5	+1.0	+1.2
pta	−2.0	+1.3	−2.4	+1.9
ackA	+2.4	+5.8	+2.3	+10.8
adhE	+3.9	−1.7	+5.8	+2.6
adhA	+2.7	−2.4	+2.6	+1.9
sdhA	+1.0	+1.5	+2.1	+3.4
citZ	−2.7	+3.2	+1.4	+2.4
citB	+1.4	+1.0	+2.6	+1.6
mdh	−1.3	+1.1	+2.1	+1.3
Enterotoxin gene nhe	−166	+1.8	−12.6	+2.4
Regulator genes
plcR	−2.2	+1.2	−1.6	+1.6
resD	−1.3	+1.2	+1.2	+2.6
fnr	−1.2	−1.1	+1.4	+1.1
ccpA	−1.1	+1.1	+1.1	+4.8

^aB. cereus cells were grown under aerobic and anaerobic conditions in controlled batch cultures (pH 7.2) on MOD medium with 30 mM glucose as the carbon source.

^bEach change (n-fold) represents the mean value of the mRNA levels of one mutant strain sample in relation to that of the parent strain, F4430/73. For each experiment, two measurements from two independent RNA samples taken from the mid-exponential growth phase (μ_max) of the same culture were analyzed in parallel. Each data point is an average of the results of the combined experiments. Only ratios of ≤−2 and ≥2 were considered significant (i.e., P ≤ 0.05) according to the precision of the method. + and − indicate up- and downregulation of genes, and significant values for upregulation and downregulation are underlined and boldface, respectively. ND, not detectable.