TABLE 1.
Corynebacterium glutamicum strains and plasmids
| Designation | Features or usea | Source or reference |
|---|---|---|
| Strains | ||
| ATCC 13032 | WT; biotin auxotroph | Culture collection |
| Ser4 | l-Serine producer; WTΔsdaA ΔpabABC (pserABC); Tetr | 37 |
| DM1933 | l-Lysine producer; WTΔpck pyc(P458S) hom(V59A) 2×lysC(T311I) 2×lysE 2×asd 2×dapA 2×dapB 2×ddh 2×lysA | 3 |
| ORN1 | l-Ornithine producer; WTΔargR ΔargF; arginine auxotroph | 13, 32 |
| WTΔargR | Deletion of transcriptional regulator of arginine synthesis | This institute |
| ARG2 | WTΔargR [pVWEx2-argB(A49VM54V)]; Tetr | This study |
| WTΔlysEG | Deletion of lysine exporter and its regulator | 40 |
| Plasmids | ||
| pEKEx2 | Shuttle vector; Kanr Ptac lacIq | 8 |
| pEKEx3 | Derived from pEKEx2; Specr | 11 |
| pVWEx2 | Derived from pEKEx2; Tetr Ptac lacIq | 8 |
| pEKE3-argB(A49VM54V) | With feedback-resistant acetylglutamate kinase; Specr | 13, 32 |
| pEKEx3-ArgR | Carrying the racemase gene of P. taetrolens with its signal sequence deleted | This study |
| pEKEx3-SP-ArgR | Carrying the full-length racemase gene of P. taetrolens | This study |
| pEKEx2-TorA-MalE | Vector providing the torA signal sequence for Tat-dependent expression | F. Lausberg; this institute |
| pEKEx2-TorA-ArgR | Replacement of the signal sequence of P. taetrolens argR by the torA signal sequence | This study |
| pVWEx2-argB(A49VM54V) | With feedback-resistant acetylglutamate kinase; Tetr | 13, 32 |
| pET22b(+)argR | Carrying the racemase gene of P. taetrolens with its signal sequence deleted and with C-terminal His6 | 22 |
a
WT, wild type; 2×, two copies.