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. 2011 Jan 28;193(7):1576–1582. doi: 10.1128/JB.00924-10

FIG. 1.

FIG. 1.

Distribution of the MalE-NmC PST chimera, after PAGE of purified MalE-NmC PST. The ultracentrifugation pellet (lane 1), supernatant of a cell lysate of AD202(pWV243) (lane 2), and the major protein fractions eluted from the amylase column (lane 3) were loaded onto a 4-to-20% polyacrylamide SDS-PAGE gel. For Western blot analysis another gel was loaded with membrane fraction (lane A), the ultracentrifuge supernatant fraction of the BL21(pWV243) lysate (lane B), and the amylose-purified MalE-NmC PST (lane C). The gel was transferred to nitrocellulose, and the immunoblot was developed with anti-MalE antibody.