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Competition of the PST elongation reaction with unlabeled acceptors. Trisialylated lactosyl-BODIPY (2.5 μM; dotted trace) was incubated with NmC PST (30 μg/ml) in the absence (black trace) or presence of 5 μM α-2,8-sialic acid trimer (maroon trace), α-2,8-hexamer (purple trace), or GD3 (blue trace).
