TABLE 1.
Plasmid constructs
| Plasmid | Gene(s) | Primer | Sequence |
|---|---|---|---|
| pWV234 | synE-nusA | CPST LIC for | GACGACGACAAGATGTTGCAGAAAATAAGA |
| CPST LIC rev | GAGGAGAAGCCCGGTTTAATTGGTTACAAAGGC | ||
| pWV235 | synE-SUMO | CPST-SUMO Met for | ATGTTGCAGAAAATAAGAAAA |
| CPST-SUMO rev | TTATTGGTTACAAAGGCTATA | ||
| pWV241 | synE | NmCPST fwd pENTR TOPO | CACCATGTTGCAGAAAATAAGAAAAGCT |
| NmCPST BamHI rev | TTTGGATCCTTATTGGTTACAAAGGCTATATTT | ||
| pWV237 | synD | NmB directional for | CACCATGCTAAAGAAAATAAAAAAAGC |
| NmB rev HindIII | AAGCTTTTATCTATCTCTACCAATTCT | ||
| pWV238 | synD | NmB directional for | CACCATGCTAAAGAAAATAAAAAAAGC |
| NmB rev HindIII | AAGCTTTTATCTATCTCTACCAATTCT | ||
| pWV245 | synD-synE | DSnmB FOR | GAGGAAAACCTGTATTTTCAGGGCT |
| DSnmB REV | TAAATGAGTCGTAATATTCTTTTTTTTTGC | ||
| pWV246 | synE-Halo tag | NmC PST Flexivector forward | AGGAGCGATCGCCATGTTGCAGAAAATAAGAAA |
| NmC PST Flexivector reverse | AACTGTTTAAACTTATTGGTTACAAAGGCTAT | ||
| pWV248 | synE | NmCPST fwd pENTR TOPO | CACCATGTTGCAGAAAATAAGAAAAGCT |
| MengC PST rev primer | TTATTGGTTACAAAGGC | ||
| pWV249 | synD-synE | B322 primer | GCGGGTCTCTTTTTTAGGAGTTATATTATT |
| C322 primer | GCGGGTCTCAAAAAATTCCTATATCCAGC | ||
| 151 primer C | GCGGGTCTCCATTCTACGGAAAACCTGTA | ||
| 151 primer D | CCCAGTGCTGCAATGATACCGCGAGA | ||
| pWV250 | synD-synE | QCL NmC322 sense primer | CCTAATAATATAACTCCTAAAAAATTCCTATATATCCAGCGTGGATATAAGA |
| QCL NmC322 antisense primer | TCTTATATCCACGCTGGATATATAGGAATTTTTTAGGAGTTATATTATTAGG |