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Acceptor activity of OMV (synD) and phenol-extracted OMV
| Acceptor | Polysialyltransferase | Acceptor activity (cpm)a |
|---|---|---|
| NmC PS | NmC PST | 36,460 |
| NmC PS | Boiled PST | 165 |
| OMV (synD) | NmC PST | 64,320 |
| OMV (phenol extract) | 20,884 | |
| C18 elution solvent | ||
| Aqueous | 920 | |
| 10% CH3CN | 18,282 | |
| 30% CH3CN | 19,092 | |
| 50% CH3CN | 1,451 |
Acceptors were incubated with the polysialyltransferase, and the formation of 14C-labeled polysialic acid was assayed as described in Materials and Methods.
