Table 1.
Analysis of 5 diagnostic tests used in the evaluation of 65 patients with 85 lesions suspected to be cutaneous leishmaniasisd
| Assay | No. of lesions with indicated result |
Sensitivity [% (95% CI)] | Specificity [% (95% CI)] | PPV (%) | NPV (%) | |
|---|---|---|---|---|---|---|
| Positive | Negative | |||||
| LSTa | 33 | 30 | 60.7 (49–72.4) | 83.3 (66.1–100) | 93.9 | 33.3 |
| Smear | 33 | 52 | 49.3 (37.3–61.3) | 100.0 | 100.0 | 34.6 |
| kDNA PCR of invasive specimensb | 64 | 21 | 92.5 (86.2–98.8) | 88.9 (74.4–100) | 96.9 | 76.2 |
| kDNA PCR of lesion aspirates | 55 | 30 | 80.6 (71.1–90.1) | 94.4 (83.8–100) | 98.2 | 56.7 |
| kDNA PCR of lesion scrapings | 54 | 31 | 79.1 (69.4–88.8) | 94.4 (83.3–100) | 98.1 | 54.8 |
| kDNA PCR of noninvasive specimensc | 60 | 25 | 86.6 (78.4–94.8) | 88.9 (74.4–100) | 96.7 | 64.0 |
| Culture | 22 | 63 | 32.8 (21.6–44) | 100.0 | 100.0 | 28.6 |
a
LST, leishmanin skin test. Two individuals did not undergo leishmanin skin testing.
b
Includes pooled analysis of lesion aspirates and scrapings.
c
Includes filter paper lesion impressions.
d
CI, confidence interval; NPV, negative predictive value; PPV, positive predictive value.