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. 2001 Feb 27;98(6):3471–3476. doi: 10.1073/pnas.051484198

Figure 1.

Figure 1

PreMBP and ProOmpA variants used in this study. The sequence of the N terminus of preMBP and proOmpA and the leader peptidase cleavage site (arrow) is shown. The point mutations, substitutions, and insertions in each variant are underlined. TM-MBP and TM-OmpA do not have a cleaved signal peptide. The proOmpA analyzed herein contains a proline in place of the naturally occurring glutamine at position −2 (italics). This change was introduced to create a restriction site that could be used to make the proOmpA variants.