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Protease-sensitivity of TM-MBP and TM-OmpA. TST1 transformed with pJH28, pJH29, pHL11, or pHL12 were pulse-labeled, converted to spheroplasts, and treated with proteinase K. MBP- and OmpA-containing polypeptides were immunoprecipitated with anti-MBP and anti-HA antibodies, respectively. Lanes: 1 and 2, cells producing MBP or OmpA; 3 and 4, cells producing TM-MBP or TM-OmpA. Proteinase K was added to the samples in lanes 1 and 3.
