Skip to main content
. 2001 Feb 27;98(6):3471–3476. doi: 10.1073/pnas.051484198

Figure 3.

Figure 3

Replacement of the MBP and OmpA signal peptides with a TMS reroutes the proteins into the SRP targeting pathway. (A) HDB51 (PBAD-ffh secB+) and HDB52 (PBAD-ffh secB−) transformed with pJH28, pJH29, pHL11, or pHL12 were grown in M9 medium containing arabinose (lanes 1–4) or glucose (lanes 5–8), radiolabeled, converted to spheroplasts, and treated with proteinase K. MBP- and OmpA-containing polypeptides and CAT were immunoprecipitated. Lanes: 1, 2, 5, and 6, HDB51 cells; 3, 4, 7, and 8, HDB52 cells. Proteinase K was added to the samples shown in lanes 1, 3, 5, and 7. (B) HDB50 and HDB51 transformed with pJH29 were grown in M9 medium containing glucose. The experiment shown in A was repeated except that 1% Triton X-100 was added to the samples in lanes 1 and 4 immediately before proteinase K treatment. Lanes: 1–3, HDB51 cells; 4–6, HDB52 cells. Proteinase K was added to the samples in lanes 1, 2, 4, and 5.