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Increasing the distance between the signal peptide and the mature region of proOmpA does not change its targeting pathway. TST1 and HDB50 (secB−) transformed with pHL11, pHL13, or pHL14 were radiolabeled, and OmpA-containing polypeptides were immunoprecipitated with anti-HA antibodies. The precursor encoded on each plasmid is indicated at the top. Lanes: 1, 3, and 5, TST1 cells; 2, 4, and 6, HDB50 cells.
