Table 2.
Comparison of the IS6110 real-time PCR and BD MGIT TBc identification tests to culture confirmation tests
| Mycobacterial species identified using biochemical methods and hsp65 PCR-RFLPa (no. of specimens) | IS6110 real-time PCR (no. of specimens = 210) |
BD MGIT TBc identification test (no. of specimens = 210) |
||
|---|---|---|---|---|
| Positive | Negative | Positive | Negative | |
| M. tuberculosis (171) | 171 | 0 | 169 | 2 |
| NTM (39) | 0 | 39 | 0 | 39 |
| M. abscessus (19) | 19 | 19 | ||
| M. kansasii (6) | 6 | 6 | ||
| M. gordonae (4) | 4 | 4 | ||
| M. intracellulare (3) | 3 | 3 | ||
| M. arupense (1) | 1 | 1 | ||
| M. avium (1) | 1 | 1 | ||
| M. chelonae (1) | 1 | 1 | ||
| M. fallax (1) | 1 | 1 | ||
| M. fortuitum (1) | 1 | 1 | ||
| M. holsaticum (1) | 1 | 1 | ||
| M. nonchromogenicum (1) | 1 | 1 | ||
a
A total of 222 specimens were primary positive in liquid culture, 11 were culture negative on L-J medium, and 1 was contaminated.