Table 4.
Summary of the performance of MPB64-based lateral-flow immunochromatographic assays in the detection of Mycobacterium tuberculosis in different studies
| Category | Study method | No. of NTM with positive results | Species (no.) | No. of MTB with negative results | Mutation(s) in mpb64 gene (no. of isolates) | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Reference | Country(ies) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Capilia TB assay | 0 | 3 | 63-bp deletion at 196 (3), 1-bp deletion at 266 (1), G→A at 402 (2), IS6110 insertion at 501 (1), 176-bp deletion at 512 (5) | 99.2 | 100 | 14 | Japan | |||
| Accuprobe | 0 | 0 | 100 | 100 | |||||||
| 2 | Capilia TB assay | 0 | 3 | C insertion at 287 (1), A→T at 388 (1), IS6110 insertion at 177 (1) | 92.4 | 100 | 13 | Germany | |||
| 3 | Capilia TB assay | 2 | MAC (1), M. chelonae (1) | 2 | 98.6 | 97.9 | 98.6 | 97.9 | 27 | Taiwan | |
| BD ProbeTec ET system | 2 | M. fortuitum (1), M. triviale (1) | 3 | 97.3 | 97.1 | 98.2 | 95.8 | ||||
| 4 | Capilia TB assay | 1 | MTBC and NTM mix | 6 | 63-bp deletion at 196 (5), 2-bp insertion at 436 (1) | 97 | 100 | 23 | Thailand | ||
| 5 | Capilia TB assay | 1 | M. intracellulare (1) | 5 | 96.9 | 98.6 | 99.4 | 93.5 | 24 | Taiwan | |
| BD ProbeTec ET system | 2 | M. intracellulare (1), M. terrae (1) | 1 | 99.4 | 97.3 | 98.8 | 98.6 | ||||
| 6 | MGIT TBc ID Test | 0 | 0 | 100 | 100 | M. Warns et al., presented at the 30th Annual Congress of the European Society of Mycobacteriology, Porto, Portugal, July 2009 | |||||
| Capilia TB assay | 1 | M. marinum (1) | 0 | 100 | 94 | ||||||
| SD bioline TB Ag MPT64 Rapid Test | 1 | M. gastri (1) | 0 | 100 | 94 | ||||||
| 7 | Capilia TB assay | 2 | M. gordonae (1), unidentified NTM (1) | 1 | GC insertion (1) | 99.6 | 99.5 | 7 | Zambia, South Africa | ||
| 8 | MGIT TBc ID Test | 0 | 2 (from 1 patient) | 63-bp deletion at 196, downstream 50 A→G (2) | 98.8 | 100 | 100 | 95.1 | This study | Taiwan | |
| IS6110 real-time PCR | 0 | 0 | 100 | 100 | 100 | 100 |