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. 2011 Mar;49(3):932–940. doi: 10.1128/JCM.02271-10

Fig. 4.

Fig. 4.

Dual Tm codes of the clinical M. tuberculosis DNA tested in this study. The Tm values of each SMB are shown for 92 clinical M. tuberculosis isolates ordered by mutation and gyrA(95S or 95T) allele. Each QRDR sequence type is seen to generate distinct dual Tm profiles. (A) Tm codes of homogeneous DNA samples. Samples 1 to 46, wild-type gyrA(95T); samples 47 to 50, wild-type gyrA(95S); samples 51 and 52, A90V gyrA(95S) mutant; samples 53 to 63, A90V gyrA(95T) mutant; samples 64 to 68, S91P gyrA(95T) mutant; samples 69 to 77, D94G gyrA(95T) mutant; sample 78, G88A gyrA(95T) mutant; sample 79, D94N gyrA(95T) mutant; sample 80, D94A gyrA(95T) mutant; sample 81, D94G gyrA(95S) mutant; sample 82, D94H gyrA(95S) mutant; and samples 83 and 84, A90V D94G gyrA(95T) double mutant. (B) Tm codes of the mixed clinical samples. Either one or both probes can be seen to have generated double peaks with distinct Tm values. Sample 1, wild type and D94A mutant; samples 2 and 4, wild type and A90V mutant; sample 3, wild type and A90V and S91P mutants; sample 5, wild type and D89N mutant; sample 6, D94A and D94G mutants; samples 7 and 8, wild type and D94N mutant. All of the samples contained the gyrA(95T) allele.