Table 1.
Two Tm codes for the clinical isolate DNA and their concordance with DNA sequence analysis
| Sample no. |
Tm value (°C) by: |
QRDR type bya: |
||
|---|---|---|---|---|
| QDR1-FAM | QDR2-Cy5 | Tm prediction | Sequencing data | |
| 1 | 64.6 | 63.2 | WT T | WT T |
| 2 | 64.6 | 63.2 | WT T | WT T |
| 3 | 64.6 | 63.3 | WT T | WT T |
| 4 | 64.5 | 63.1 | WT T | WT T |
| 5 | 64.6 | 63.2 | WT T | WT T |
| 6 | 64.5 | 63.5 | WT T | WT T |
| 7 | 64.5 | 63.3 | WT T | WT T |
| 8 | 64.6 | 63.3 | WT T | WT T |
| 9 | 64.5 | 63.1 | WT T | WT T |
| 10 | 64.9 | 63.8 | WT T | WT T |
| 11 | 64.5 | 63.2 | WT T | WT T |
| 12 | 64.5 | 63.2 | WT T | WT T |
| 13 | 64.6 | 63.4 | WT T | WT T |
| 14 | 64.5 | 63.1 | WT T | WT T |
| 15 | 64.4 | 63.2 | WT T | WT T |
| 16 | 64.4 | 63.2 | WT T | WT T |
| 17 | 64.6 | 63.3 | WT T | WT T |
| 18 | 64.5 | 63.2 | WT T | WT T |
| 19 | 64.6 | 63.3 | WT T | WT T |
| 20 | 64.5 | 63.3 | WT T | WT T |
| 21 | 64.4 | 63.0 | WT T | WT T |
| 22 | 64.4 | 63.8 | WT T | WT T |
| 23 | 64.6 | 63.2 | WT T | WT T |
| 24 | 64.4 | 63.2 | WT T | WT T |
| 25 | 64.3 | 63.0 | WT T | WT T |
| 26 | 64.3 | 63.1 | WT T | WT T |
| 27 | 64.4 | 63.1 | WT T | WT T |
| 28 | 64.3 | 63.0 | WT T | WT T |
| 29 | 64.3 | 62.9 | WT T | WT T |
| 30 | 64.4 | 63.2 | WT T | WT T |
| 31 | 64.3 | 63.1 | WT T | WT T |
| 32 | 64.3 | 63.0 | WT T | WT T |
| 33 | 64.3 | 63.1 | WT T | WT T |
| 34 | 64.4 | 63.2 | WT T | WT T |
| 35 | 64.3 | 63.0 | WT T | WT T |
| 36 | 64.6 | 63.2 | WT T | WT T |
| 37 | 64.3 | 62.9 | WT T | WT T |
| 38 | 64.4 | 62.9 | WT T | WT T |
| 39 | 64.4 | 62.9 | WT T | WT T |
| 40 | 64.3 | 62.8 | WT T | WT T |
| 41 | 64.2 | 62.9 | WT T | WT T |
| 42 | 64.3 | 62.8 | WT T | WT T |
| 43 | 64.3 | 62.9 | WT T | WT T |
| 44 | 64.3 | 62.8 | WT T | WT T |
| 45 | 64.3 | 62.8 | WT T | WT T |
| 46 | 64.3 | 62.9 | WT T | WT T |
| 47 | 61.3 | 61.3 | WT S | WT S |
| 48 | 61.4 | 61.3 | WT S | WT S |
| 49 | 61.6 | 61.5 | WT S | WT S |
| 50 | 61.3 | 61.5 | WT S | WT S |
| 51 | 61.6 | 57.3 | G88A T | G88A T |
| 52 | 65.5 | 65.4 | A90V S | A90V S |
| 53 | 65.7 | 65.4 | A90V S | A90V S |
| 54 | 68.3 | 66.9 | A90V T | A90V T |
| 55 | 68.3 | 66.9 | A90V T | A90V T |
| 56 | 68.3 | 66.9 | A90V T | A90V T |
| 57 | 68.3 | 67.3 | A90V T | A90V T |
| 58 | 68.3 | 66.7 | A90V T | A90V T |
| 59 | 68.2 | 66.6 | A90V T | A90V T |
| 60 | 68.3 | 66.7 | A90V T | A90V T |
| 61 | 68.2 | 66.7 | A90V T | A90V T |
| 62 | 68.5 | 66.8 | A90V T | A90V T |
| 63 | 68.3 | 66.7 | A90V T | A90V T |
| 64 | 68.3 | 66.7 | A90V T | A90V T |
| 65 | 58.8 | 58.6 | S91P T | S91P T |
| 66 | 58.6 | 58.3 | S91P T | S91P T |
| 67 | 58.6 | 58.2 | S91P T | S91P T |
| 68 | 58.6 | 58.3 | S91P T | S91P T |
| 69 | 58.5 | 58.3 | S91P T | S91P T |
| 70 | 66.2 | 59.1 | D94G S | D94G S |
| 71 | 68.3 | 60.6 | D94G T | D94G T |
| 72 | 68.4 | 60.5 | D94G T | D94G T |
| 73 | 68.4 | 60.5 | D94G T | D94G T |
| 74 | 68.3 | 60.5 | D94G T | D94G T |
| 75 | 68.3 | 60.5 | D94G T | D94G T |
| 76 | 68.5 | 60.7 | D94G T | D94G T |
| 77 | 68.2 | 60.3 | D94G T | D94G T |
| 78 | 68.3 | 60.4 | D94G T | D94G T |
| 79 | 68.2 | 60.3 | D94G T | D94G T |
| 80 | 62.9 | 57.4 | D94H/N/Y T | D94N T |
| 81 | 64.2 | 58.6 | G88C T or D94A T | D94A T |
| 82 | 60.0 | 54.5 | G88C S or D94H/N/Y S | D94H S |
| 83 | 71.7 | 64.0 | New mutant/double mutant | A90V T D94G T |
| 84 | 69.8 | 63.3 | New mutant/double mutant | A90V T D94G T |
| 85 | 63.6 | 57.7–63.5 | WT T + G88C or D94A T | WT T + D94A T |
| 86 | 63.7–68.0 | 62.5–67.3 | WT T + A90V T | WT T + A90V T |
| 87 | 59.1–64.6 | 58.6–63.3 | WT T + S91P T | WT T + A90V + S91P T |
| 88 | 64.2–68.5 | 63.12–66.9 | WT T + A90V T | WT T + A90V T |
| 89 | 58.5–64.5 | 62.9 | Mixed DNA | WT T + D89N T |
| 90 | 65.4–69.9 | 59.8 | Mixed DNA | D94G T + D94A T |
| 91 | 64.2 | 57.6–63.3 | WT T + D94H/N/Y T | WT T + D94N T |
| 92 | 63.0 | 57.5–63.3 | WT T + D94H/N/Y T | WT T + D94N T |
a
WT, wild type; T, 95T allele (threonine); S, 95S allele (serine).