Table 2.
Post-PCR Tm values with artificial templates harboring QRDR mutationsa
| QRDR typeb | QDR1 |
QDR2 |
||
|---|---|---|---|---|
| Tm (°C) | dTm (°C)c | Tm (°C) | dTm (°C)c | |
| WT T | 66.2 | 65.4 | ||
| G88A T | 61.7 | 4.4 | 58.1 | 7.4 |
| G88C T | 66.0 | 0.2 | 60.5 | 5.0 |
| D89N T | 60.3 | 5.9 | 58.3 | 7.2 |
| A90V T | 69.8 | −3.6 | 68.0 | −2.6 |
| S91P T | 61.3 | 4.9 | 62.6 | 2.8 |
| D94A T | 66.1 | 0.1 | 60.3 | 5.1 |
| D94G T | 70.3 | −4.2 | 63.5 | 1.9 |
| D94H T | 65.0 | 1.2 | 58.8 | 6.6 |
| D94N T | 64.9 | 1.3 | 58.8 | 6.6 |
| D94Y T | 64.8 | 1.4 | 58.2 | 7.3 |
| WT S | 63.8 | 64.6 | ||
| G88A S | 59.0 | 4.8 | 57.4 | 7.3 |
| G88C S | 63.5 | 0.3 | 57.4 | 7.2 |
| D89N S | 57.2 | 6.6 | 58.4 | 6.2 |
| A90V S | 67.5 | −3.8 | 67.8 | −3.2 |
| S91P S | 58.9 | 4.9 | 59.3 | 5.3 |
| D94A S | 63.9 | −0.1 | 59.3 | 5.4 |
| D94G S | 68.3 | −4.6 | 62.0 | 2.7 |
| D94H S | 62.5 | 1.3 | 56.7 | 8.0 |
| D94N S | 62.5 | 1.3 | 56.6 | 8.0 |
| D94Y S | 62.4 | 1.4 | 56.6 | 8.0 |
a
Results represent the average of 10 separate reactions. Tm values did not differ by more than 0.5 to 0.8°C.
b
WT, wild type; T, 95T allele (threonine); S, 95S allele (serine).
c
dTm = WT Tm − mutant Tm. Negative dTm values imply a higher Tm for the probe with the mutant sequence than that for the wild-type sequence.